CiteScore: 1.9     h-index: 21

Document Type : Original Research Article


1 Department of Biochemistry, Payame Noor University, 19395- 4697, Tehran, Iran

2 Department of Biochemistry, Payame Noor University, 81581-84431, Isfahan, Iran


Phycocyanin is a blue pigment and water-soluble biliprotein from the spirulina platensis. It has great medical and medicinal properties and has been used as a nutritional supplements. Phycocyanin is also a natural and powerful antioxidant and anti-inflammatory. In this study, the phycocyanin of Spirulina platensis was extracted by using two enzymatic and ultrasonic methods and purified by ammonium sulfate precipitation and dialysis. The UV spectrophotometer absorption of the extracted sample showed a broad peak range at 280, 615, and 652 nm. By using The FT-IR results confirmed the structure and molecular bonds from extracted and purificated phycocyanin. The concentrations obtained at the enzymatically was 0.405 mg/mL and for sonication 0.422 mg/mL. By using the SDS PAGE method, bands of phycocyanin were identified and the molecular weight was determined between 19-14 KD. The antioxidant and anti-inflammatory activity of the phycocyanin was approved with the reaction by DPPH and HOCl.

Graphical Abstract

Extraction and purification of phycocyanin from spirulina platensis and evaluating its antioxidant and anti- inflammatory activity


Main Subjects

[1]. Shams A., Nmtzadeh G., Soltani N., Shokravi H. J. Envir. Phys. Plant., 2015, 38:2
[2]. Kumar D., Dhar D.W., Pabbi S., Kumar N., Walia S. Ind J Plant Physiol., 2014, 19:184
[3]. Chu W.L., Lim Y.W., Radhakrishnan A.K. The official journal of the International Society for Complementary Medicine Research, 2010, 10:53
[4]. Brient L., Lengronne M., Bertrand E., Rolland D., Sipel A., Steinmann D., Baudin I., Legeas M., Le Rouzic B., Bormans M. Journal of Environmental Monitoring, 2008, 10:248
[5]. Jerley A.A., Prabu D.M. Journal of Agriculture, Plant Biotechnology and Bio Products (SIRJ-APBBP), 2015, 2:1
[6]. Penton Rolb G., Marin Pridaa J., Pardo Andreua G., Martinez Sancheza G., Felino E., Medinaa Alain A., Acostaa V., Lagumersindez Denisa N., Rodriguez Jimenezb E., Llopiz Arzuagab A., Lopez A., Saurab P., Guillen Nietob G., Penton Ariasb E. Brain Research Bulletin., 2011, 86:42
[7]. Bermejo R., Talavera E.M., Alvarez-Pez J.M., Orte J.C. J. Chromatogr. A, 1997, 778:441
[8]. Song W., Zhao C., Wang S. Int. J. Biosci. Biochem. Bioinforma., 2013, 3:293
[9]. Sarada R., Pillai M.G., Ravishankar G.A. Process Biochemistry, 1999, 34:795
[10]. Romay Ch., González R., Ledón N., Remirez D., Rimbau V. Current Protein and Peptide Science, 2003, 4:207
[11]. Bhat V.B,. Madyastha K.M. Biochem. Biophys. Res. Commun., 2000. 275:20
[12]. McCarty M.F. J. Med. Food., 2007, 10:566
[13]. Terry M.J., Maines M.D., Lagarias J.C. J. Biol.  Chem., 1993. 268:106
[14]. Geiszt M. Cardiovascular Research, 2006, 71:289
[15]. Paravicini T.M., Touyz R.M. Diabetes Care., 2008., 31:170
[16]. Neurochem J. Journal of Neurochemistry, 2016. 1:318
[17]. Sharma N., Kapoor M., Nehru B. Behav. Brain Res., 2016, 296:177
[18]. Pabon M.M., Jernberg J.N., Morganti J., Contreras J., Hudson C.E., Klein R.L., Bickford P.C.
PLoS One, 2012, 7:e45256
[19]. Hare J.M., Beigi F., Tziomalos K. Methods. Enzymol., 2008, 441:369
[20]. Romay C., Gonzalez R., Pizarro M., Lissi E. J. Protein Chem., 2000, 19:151
[21]. Talebi R., Alimoradian A., Sadegh M. Arak University of Medical Journal, 2015, 18:33
[22]. Prabakaran P., David Ravindran A. International Journal of Research in Pharmacy and Life Sciences, 2013, 1:15
[23]. Abd El-Baky H.H., El Baz F.K., El-Baroty G.S. J. Medic. Plant. Res., 2008, 2:292
[24]. Zhang X., Zhang F., Luo G., Yang Sh., Wang D. J. Food Nutr. Res., 2015, 3:15
[25]. Chu W.L., Lim Y.W., Radhakrishnan A.K., Lim P.E. Journal of the International Society for Complementary Medicine Research, 2010, 10:53
[26]. Cifuentes-Pagano E., Csanyi G., Pagano P.J. HHS Public Access, 2015, 69:2315