TY - JOUR ID - 63597 TI - Extraction and purification of phycocyanin from spirulina platensis and evaluating its antioxidant and anti- inflammatory activity JO - Asian Journal of Green Chemistry JA - AJGC LA - en SN - 2588-5839 AU - Izadi, Mahdieh AU - Fazilati, Mohammad AD - Department of Biochemistry, Payame Noor University, 19395- 4697, Tehran, Iran AD - Department of Biochemistry, Payame Noor University, 81581-84431, Isfahan, Iran Y1 - 2018 PY - 2018 VL - 2 IS - 4 SP - 364 EP - 379 KW - Phycocyanin KW - Spirulina platensis KW - NADPH Oxidase DO - 10.22034/ajgc.2018.63597 N2 - Phycocyanin is a blue pigment and water-soluble biliprotein from the spirulina platensis. It has great medical and medicinal properties and has been used as a nutritional supplements. Phycocyanin is also a natural and powerful antioxidant and anti-inflammatory. In this study, the phycocyanin of Spirulina platensis was extracted by using two enzymatic and ultrasonic methods and purified by ammonium sulfate precipitation and dialysis. The UV spectrophotometer absorption of the extracted sample showed a broad peak range at 280, 615, and 652 nm. By using The FT-IR results confirmed the structure and molecular bonds from extracted and purificated phycocyanin. The concentrations obtained at the enzymatically was 0.405 mg/mL and for sonication 0.422 mg/mL. By using the SDS PAGE method, bands of phycocyanin were identified and the molecular weight was determined between 19-14 KD. The antioxidant and anti-inflammatory activity of the phycocyanin was approved with the reaction by DPPH and HOCl. UR - https://www.ajgreenchem.com/article_63597.html L1 - https://www.ajgreenchem.com/article_63597_3db16ccfc57719527e7bfb36b1362631.pdf ER -