Asian Journal of Green Chemistry
3.8(Q2)
CiteScore
27
h-index

In Vitro Screening of Anticancer Activity of Dregea volubilis and Leptadenia reticulata Using Sulforhodamine B (SRB) Assay

Document Type : Original Research Article

Authors

Venkatesan Natarajan 1
Harish Nagaraju 2
Chandan Sathish Kumar 3
Anusha Ramesh 4
K Shivaraj 5
Aravinth Velmurugan 6
Thamizh Senthamarai Kannan 7

1 Department of Pharmacology, School of Pharmacy, Sri Balaji Vidyapeeth (Deemed to be University), Pondicherry - 607402, India

2 Department of Pharmacology, RR College of Pharmacy, Bangalore – 560090, India

3 Department of Pharmacology, Aditya Bangalore Institute of Pharmacy Education and Research, Bangalore -560064, India

4 Department of Pharmacology, MVM College of Pharmacy, Bangalore – 560064, India

5 Department of Pharmacology, Nandi Medical College and Research Institute, Ckikkaballapur, Karnataka, India

6 Department of Pharmacognosy, School of Pharmacy, Sri Balaji Vidyapeeth (Deemed to be University), Pondicherry-607402, India

7 Department of Pharmaceutical Chemistry, School of Pharmacy, Sri Balaji Vidyapeeth (Deemed to be University), Pondicherry – 607402, India

https://doi.org/10.48309/ajgc.2026.553231.1845
Abstract
The present study aimed to evaluate the anticancer potential of Dregea volubilis and Leptadenia reticulata extracts using the Sulforhodamine B (SRB) assay against selected human cancer cell lines. Ethanolic extracts of both plants were tested, and Adriamycin was used as the reference standard. The GI₅₀ values of Dregea volubilis and Leptadenia reticulata extracts were found to be 62.4 µg/mL and 78.6 µg/mL, respectively, while Adriamycin exhibited a GI₅₀ value of 1.2 µg/mL under similar conditions, indicating comparatively lower potency of the plant extracts. Among the two, Dregea volubilis showed better cytotoxic activity. The findings suggest that the bioactive constituents of these plants may serve as leads for the development of novel anticancer agents. This study provides the first comparative evidence of the anticancer potential of these traditional medicinal plants, supporting their ethnopharmacological relevance.

Graphical Abstract

In Vitro Screening of Anticancer Activity of Dregea volubilis and Leptadenia reticulata Using Sulforhodamine B (SRB) Assay

Keywords

Dregea volubilis
Leptadenia reticulata
SRB assay
In vitro cytotoxicity
Anticancer activity
GI₅₀

Subjects

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Asian Journal of Green Chemistry
Volume 10, Issue 3
May and June 2026
Pages 500-507

  • Receive Date 14 October 2025
  • Revise Date 27 November 2025
  • Accept Date 22 December 2025

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