Asian Journal of Green Chemistry
3.8(Q2)
CiteScore
27
h-index

Extraction and isolation of anti-hypertensive peptide by alkalase from spirulina platensis

Document Type : Original Research Article

Authors

Gholamreza Mahdieh
Mohamad Fazilati
Mahdieh Izadi

Department of Biochemistry, Payame Noor University, 19395- 4697, Tehran, Iran

https://doi.org/10.22034/ajgc.2018.65318
Abstract
Spirulina has attracted a great attention as it contains many nutrients, such as protein, vitamins and minerals. Spirulina contains peptides that have therapeutic and beneficial effects on the human body. Some medicinal properties of the biological peptides of Spirulina platensis include antioxidants, antifungal, antimicrobial, anti-diabetes and anticoagulant activity. This study provides an overview of the biological peptides derived from Spirulina and some biological activities with health benefits. In this study peptide Ile-Gln-Pro from spirulina platalsis was isolated using an alkalase enzyme and investigate its inhibitory effect on the angiotensin I-converting enzyme (ACE). This peptide was purified by gel filtration chromatography and Reverse-phase high-performance liquid chromatography (RP-HPLC) Enzymatic kinetic studies showed a non-competitive inhibitory activity of this peptide, and the Ki value was 5.8 μm.

Graphical Abstract

Extraction and isolation of anti-hypertensive peptide by alkalase from spirulina platensis

Keywords

Spirulina platensis
Alkalase
ACE inhibitory peptide

Subjects

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Asian Journal of Green Chemistry
Volume 3, Issue 1
Winter 2019
Pages 13-21

  • Receive Date 23 April 2018
  • Revise Date 21 May 2018
  • Accept Date 21 May 2018

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