CiteScore: 1.9     h-index: 21

Document Type : Original Research Article


B.K. Mody Government Pharmacy College, Rajkot, India


A simple, rapid, precise, and accurate isocratic reversed-phase stability indicating HPLC method was developed and validated for simultaneous determination of the nebivolol and valsartan in the tablet dosage form. The effective chromatographic separation was achieved by a YMC pack pro octadecyl silane (150×4.6 mm, 3 µm) column using a mobile phase composed of methanol: acetonitrile: 0.05 M potassium dihydrogen phosphate buffer (Ph=3.0 with 10% ortho phosphoric acid after addition of 0.2% triethylamine) (30:30:40, v/v/v) at a flow rate of 1 mL/min and UV detection at 282 nm. Drugs were subjected to the acid, base, oxidation, heat, and photolysis to apply the stress. Linearity ranges were 5–30 µg/mL (r2= 0.9989) for nebivolol and 80-480 µg/mL (r2=0.9991) for valsartan. Limit of detection was 0.38 µg/mL and 1.08 µg/mL for nebivolol and valsartan, respectively. The limit of quantification for the nebivolol and valsartan was 1.15 µg/mL and 3.27 µg/mL, respectively.

Graphical Abstract

Stability indicating assay method for simultaneous estimation of nebivolol and valsartan in pharmaceutical dosage form by RP-HPLC


Main Subjects

[1]. Chaudhary A.B., Patel R.K., Chaudhary S.A. Int. J. Res. Pharm. Sci., 2010, 1:108
[2]. Kokil S.U., Bhatia M.S. Indian J. Pharm. Sci., 2009, 71:111
[3]. Selvan P.S., Gowda K.V., Mandal U., Solomon Sam W.D., Pal T.K.  J. Chromatogr. B., 2007, 858:143
[4]. Patel L.J., Suhagia B.N., Shah P.B. Indian J. Pharm. Sci., 2007, 69:594
[5]. Ahir K.B., Patelia E.M., Mehta F.A. J. Chromat. Separation Techniq., 2012, 3:1
[6]. Ramakrishna N.V.S., Vishwottam K.N., Koteshwara M., Manoj S., Santosh M., Varma D.P. J. Pharm. and Biomed. Anal.,  2005, 39:1006
[7]. Rao K., Jena N., Rao M. J. Young Pharm., 2010, 2:183
[8]. Shah D.A., Bhatt K.K., Mehta R.S., Baldania S.L., Gandhi T.R. Indian J. Pharm.Sci., 2008, 70:591
[9]. Meyyanathan S.N., Rajan S., Muralidharan S., Birajdar A.S., Suresh B.A. Indian J. Pharm. Sci., 2008, 70:687
[10]. Li H., Wang Y., Jiang Y., Tang Y., Wang J., Zhao L., Gu J. J. Chromatogr. B., 2007, 852:436
[11]. Walsangikar S., Ghate S., Patrakar R., Deshpande A., Patil S., Gadgul A. Int. J. Drug Dev. & Res., 2010, 2:635
[12]. Nandania J., Rajput S.J., Contractor P., Vasava P., Solanki B. J. Chromatogr. B., 2013, 923-924:110
[13]. Shaalan R.A., Belal T.S., Yazbi F.A.E.I., Elonsy S.M. Arab. J. Chem., 2013, 1
[14]. Tatar S., Sag S. J. Pharm. and Biomed. Anal., 2002, 30:371