Asian Journal of Green Chemistry
3.8(Q2)
CiteScore
27
h-index

Evaluation of Anti-Asthmatic Potential of Emblica Officinalis Seed Extract in RAW 264.7 Cell Lines

Articles in Press

Document Type : Original Research Article

Authors

Elango Kannan 1
Mumtha Logesh 1, 2
Priyadharshini S 2

1 Faculty of Pharmacy, Karpagam Academy of Higher Education, Coimbatore- 641 105, Tamil Nadu, India

2 Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Ooty, Nilgiris- 643001, Tamil Nadu, India

10.48309/ajgc.2026.553890.1848
Abstract
This study aims to explore the anti-asthmatic potential of Emblica officinalis seed extract by assessing its effect on inflammatory markers in RAW 264.7 cell line. The preliminary evaluation of Emblica officinalis seed extract performed and characterized by UV, HPLC, and GCMS. Molecular docking was carried out using Schrodinger. DPPH, ABTS, and nitric oxide production were determined to study antioxidant effects, and cytotoxicity was assessed by MTT assay. The expression of IL-4, IL-13, TNF-alpha in LPS induced RAW 264.7 cell lines was analyzed using FACS method. The hydroalcoholic extract produced an extractive and ash values of 2.85% w/w and 3.47 ± 0.15% w/w, respectively. The determination of total phenolic and flavonoid content was favorable. The characterization of the extract was performed and analyzed through HPLC chromatograms. The GCMS chromatogram showed peaks at various retention times indicating the presence of phytoconstituents. In silico studies showed good interactions between selected ligands and the target proteins. The antioxidant studies proved that the extract possesses free radical scavenging activity, reduces nitric oxide production, and MDA levels. The extract proved to be non-toxic in the MTT assay. In LPS induced RAW 264.7 cells the expression of the target proteins that actively involved in the pathogenesis of asthma was decreased.

Graphical Abstract

Evaluation of Anti-Asthmatic Potential of Emblica Officinalis Seed Extract in RAW 264.7 Cell Lines

Keywords

Antiasthmatic activity
Emblica officinalis
Seed extract
RAW 264.7 cell line
LPS induced

Subjects

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Asian Journal of Green Chemistry

Articles in Press, Accepted Manuscript
Available Online from 26 January 2026

  • Receive Date 18 October 2025
  • Revise Date 21 November 2025
  • Accept Date 22 January 2026

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